Yi Huang
共找到 6 条论著文献

1、GCKGene-Body Hypomethylation Is Associated with the Risk of Coronary Heart Disease

2、An association study between genetic polymorphisms related to lipoprotein-associated phospholipase A2 and coronary heart disease

摘要:Previous genome-wide association studies (GWAS) have revealed seven single nucleotide polymorphisms (SNPs) that affect lipoprotein-associated phospholipase A2 (Lp-PLA2) activity or levels in American and European individuals. A total of 290 coronary heart disease (CHD) patients, 198 non-CHD patients and 331 unrelated healthy volunteers were recruited for the present case-control study of Han Chinese. Four SNPs (rs964184 of ZNF259, rs7528419 of CELSR2 and rs7756935 and rs1805017 of PLA2G7) were shown to be significantly associated with CHD. The rs964184-G allele of the ZNF259 gene was identified as a risk factor of CHD in females (odds ratio (OR) =1.49, 95% confidence interval (CI) =1.00-2.22, P=0.05). The rs7528419-G allele of the CELSR2 gene was protective against CHD in males (OR=0.48, 95% CI=0.25‑0.93, P=0.04). The other two alleles (rs7756935-C and rs1805017-A) of the PLA2G7 gene acted as protective factors against CHD in females (rs7756935-C: OR=0.59, 95% CI=0.35-1.00, P=0.05; rs1805017-A: OR=0.51, 95% CI=0.28-0.93, P=0.03). Moreover, rs1805017 of the PLA2G7 gene was associated with the severity of CHD only in females (r2=0.02, P=0.04). We identified four Lp-PLA2-associated SNPs significantly associated with CHD in a Han Chinese population. Specifically, rs7528419 was protective factor against CHD in males, while the other two SNPs (rs7756935 and rs1805017 of the PLA2G7 gene) were protective factors against CHD in females and rs964184 of the ZNF259 gene was regarded as a risk factor for CHD in females.
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3、Functional prediction of miR-3144-5p in human cardiac myocytes based on transcriptome sequencing and bioinformatic 2017

摘要:1 online resource Abstract: Background: RAN guanine nucleotide release factor ( RANGRF ) encoding protein MOG1 plays an important role in cardiac arrhythmia, so we intended to investigate the regulatory miRNA of RANGRF and explore its potential regulatory mechanism in arrhythmogenesis. Methods: Based on bioinformatic analysis, miR-3144-5p was predicted to be a regulatory miRNA of RANGRF, which were then validated through a dual-luciferase reporter plasmid assay. Subsequently, the expression level of miR-3144-5p in human cardiac myocytes (HCMs) was detected, followed by cell transfection with miR-3144-5p mimics. Transcriptome sequencing was then performed in HCMs with or without transfection. The sequencing results were subjected to bioinformatic analyses, including differentially expressed gene (DEG) analysis, functional enrichment analysis, protein–protein interaction (PPI) network analysis, miRNA-target gene analysis, and miRNA-transcription factor (TF)-target gene coregulatory network analysis. Results: There really existed a regulatory relation between miR-3144-5p and RANGRF . The expression level of miR-3144-5p was low in HCMs. After cell transfection, miR-3144-5p expression level significantly increased in HCMs. Bioinformatic analyses of the transcriptome sequencing results identified 300 upregulated and 271 downregulated DEGs between miR-3144-5p mimic and control group. The upregulated genes ISL1 and neuregulin 1 ( NRG1 ) were significantly enriched in cardiac muscle cell myoblast differentiation (GO:0060379). CCL21 was one of the hub genes in the PPI network and also a target gene of miR-3144-5p. Moreover, the TF of v-Myc avian myelocytomatosis viral oncogene neuroblastoma-derived homolog (MYCN) was involved in the miR-3144-5p-TF-target gene coregulatory network and interacted with the target genes of miR-3144-5p. Conclusion: ISL1, NRG1, CCL21, and MYCN were differentially expressed in the miR-3144-5p mimic group, suggesting that miR-3144-5p overexpression plays a role in HCMs by regulating these genes and TF. This study may provide new insight into the mechanisms behind the progression of cardiac arrhythmia.
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4、Failure analysis of the turret bearings wear on SYS in the Bohai Bay area

5、Retrotransposons Are the Major Contributors to the Expansion of the Drosophila ananassae Muller F Element

摘要:the discordance between genome size and the complexity of eukaryotes can partly be attributed to differences in repeat density. the muller f element (∼5.2 mb) is the smallest chromosome in drosophila melanogaster, but it is substantially larger (>18.7 mb) in d. ananassae. to identify the major contributors to the expansion of the f element and to assess their impact, we improved the genome sequence and annotated the genes in a 1.4-mb region of the d. ananassae f element, and a 1.7-mb region from the d element for comparison. we find that transposons (particularly ltr and line retrotransposons) are major contributors to this expansion (78.6%), while wolbachia sequences integrated into the d. ananassae genome are minor contributors (0.02%). both d. melanogaster and d. ananassae f-element genes exhibit distinct characteristics compared to d-element genes (e.g., larger coding spans, larger introns, more coding exons, and lower codon bias), but these differences are exaggerated in d. ananassae. compared to d. melanogaster, the codon bias observed in d. ananassae f-element genes can primarily be attributed to mutational biases instead of selection. the 5′ ends of f-element genes in both species are enriched in dimethylation of lysine 4 on histone 3 (h3k4me2), while the coding spans are enriched in h3k9me2. despite differences in repeat density and gene characteristics, d. ananassae f-element genes show a similar range of expression levels compared to genes in euchromatic domains. this study improves our understanding of how transposons can affect genome size and how genes can function within highly repetitive domains.
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6、Ultra-deep targeted sequencing of advanced oral squamous cell carcinoma identifies a mutation-based prognostic gene signature