Limin Zhao
共找到 3 条论著文献

1、Amplified Chemiluminescence Detection of DNA by Strand-Displacement Polymerization Target Recycling and G-quadruplexes/Hemin DNAzyme Catalysi

摘要:A new strategy based on strand-displacement polymerization target recycling and G-quadruplex DNAzyme catalysis was developed for amplified chemiluminescence detection of DNA. The amplified detection was achieved by using the system consisted of hairpin DNA probe, G-rich DNA, primer, and polymerase Klenow fragment exo−. When the target DNA was introduced the system, the hairpin structure of DNA probe was opened by the hybridizing of target DNA with its complementary sequence, the primer hybridized then with DNA probe and initiated polymerase-aided strand-displacement polymerization reaction, resulting in the release of target DNA and G-rich DNA. The released target DNA again hybridizes with another DNA probe to trigger the next polymerase-aided strand-displacement polymerization, generating large numbers of G-rich DNA. The G-rich DNA assembles with hemin to form the G-quadruplexes/hemin DNAzyme, which can catalyze oxidation of luminol by H2O2, generating chemiluminescence. This unique amplifying strategy gives a detection limit down to 2.5 pM, which is at least two to three orders of magnitude lower than that of unamplified DNA detection methods.
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2、Velocity Criteria for Intracranial Stenosis Revisited An International Multicenter Study of Transcranial Doppler and Digital Subtraction Angiography

3、Modeling diurnal variation of ground thermal radiance images using energy balance model and endmember composing technique

摘要:Modeling and analyzing dynamic changes of land thermal radiance scenes play an important role in thermal remote sensing. In this paper, the diurnal variation of ground surface thermal scene is mainly discussed. Firstly, based on the land surface energy balance equation, the diurnal variation of land surface temperatures (LSTs) over bare land covers were simulated by an analytical thermal model with second harmonic terms, and the diurnal LST variation of vegetation canopy was simulated using the Cupid model. Secondly, normalized difference vegetation index (NDVI), normalized difference water index (NDWI), and ratio resident-area index (RRI) were used to evaluate the endmember abundance of four land cover types including vegetation, bare soil, impervious and water area, which were calculated from IKONOS visible and near infrared (VNIR) bands. Finally, the thermal radiance scenes at various times and view angles were modeled based on the linear-energy-mixing hypothesis. The results showed that the simulated daily LST variations for vegetated and bare surfaces are correlated with the measured values with a maximum standard deviation of 2.7°C, that land thermal radiant textures with high-resolution are restored from the linear-energy-mixing method, and that the information abundance of the scene are related to the distribution of land cover, the imaging time, and the view angle.
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