Yoshinori Koga
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1、Multiple mitochondrial DNA deletions exist in cardiomyocytes of patients with hypertrophic or dilated cardiomyopathy

摘要:Genetic impairment was revealed in idiopathic cardiomyopathy and the responsible DNA locus was estimated. Mitochondrial DNA were amplified from autopsied cardiac specimens from three patients who died from hypertrophic or dilated cardiomyopathy by using polymerase chain reaction (PCR). By using two novel methods for PCR gene amplification, the pleioplasmic existence of multiple populations of differently deleted mitochondrial DNA in all specimens from the patients was confirmed. Mitochondrial DNA with a 7,436 bp deletion which commonly existed among the specimens was sequenced and the direct repeat at each edge of deletion was identified as (CATCAACAACCG) which was located in ATPase 6 gene and in the D-loop region. From our results mitochondrial DNA mutations could also be an important contributory factor to cardiomyopathy.
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2、Time-resolved study of CO elimination from Mo(CO)6in SF6-sensitized CO2laser CVD proce

摘要:The gas-phase decomposition process of Mo(CO)6 in SF6-sensitized CO2 laser chemical vapor deposition (CVD) of Mo thin films is investigated by means of the time-resolved diode-laser spectroscopic technique. The time variation of the rotational translational and vibrational temperatures of CO eliminated from Mo(CO)6 and that of the CO concentration are determined by observing several infrared absorption lines of the fundamental and hot bands of CO. The kinetics of CO elimination in the CVD process is discussed.
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