Wei Zhang
共找到 93 条论著文献

1、Abstracts from the 8th International Congress of the Asia Pacific Society of Infection Control (APSIC) Bangkok, Thailand. 12-15 February 2017

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2、Chemically derived graphene–metal oxide hybrids as electrodes for electrochemical energy storage: pre-graphenization or post-graphenization?

3、A Method to Exploit the Structure of Genetic Ancestry Space to Enhance Case-Control Studie

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4、Genomic and Functional Approaches to Understanding Cancer Aneuploidy

摘要:Aneuploidy, whole chromosome or chromosome arm imbalance, is a near-universal characteristic of human cancers. In 10,522 cancer genomes from The Cancer Genome Atlas, aneuploidy was correlated with TP53 mutation, somatic mutation rate, and expression of proliferation genes. Aneuploidy was anti-correlated with expression of immune signaling genes, due to decreased leukocyte infiltrates in high-aneuploidy samples. Chromosome arm-level alterations show cancer-specific patterns, including loss of chromosome arm 3p in squamous cancers. We applied genome engineering to delete 3p in lung cells, causing decreased proliferation rescued in part by chromosome 3 duplication. This study defines genomic and phenotypic correlates of cancer aneuploidy and provides an experimental approach to study chromosome arm aneuploidy. Analyzing >10,000 human cancers, Taylor et al. show that aneuploidy is correlated with somatic mutation rate, expression of proliferation genes, and decreased leukocyte infiltration. Loss of chromosome arm 3p is common in squamous cancers, but deletion of chromosome 3p reduces cell proliferation in vitro.
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5、Scalable Open Science Approach for Mutation Calling of Tumor Exomes Using Multiple Genomic Pipeline

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6、Spatial Organization and Molecular Correlation of Tumor-Infiltrating Lymphocytes Using Deep Learning on Pathology Image

摘要:Beyond sample curation and basic pathologic characterization, the digitized H&E-stained images of TCGA samples remain underutilized. To highlight this resource, we present mappings of tumor-infiltrating lymphocytes (TILs) based on H&E images from 13 TCGA tumor types. These TIL maps are derived through computational staining using a convolutional neural network trained to classify patches of images. Affinity propagation revealed local spatial structure in TIL patterns and correlation with overall survival. TIL map structural patterns were grouped using standard histopathological parameters. These patterns are enriched in particular T cell subpopulations derived from molecular measures. TIL densities and spatial structure were differentially enriched among tumor types, immune subtypes, and tumor molecular subtypes, implying that spatial infiltrate state could reflect particular tumor cell aberration states. Obtaining spatial lymphocytic patterns linked to the rich genomic characterization of TCGA samples demonstrates one use for the TCGA image archives with insights into the tumor-immune microenvironment. Tumor-infiltrating lymphocytes (TILs) were identified from standard pathology cancer images by a deep-learning-derived 「computational stain」 developed by Saltz et al. They processed 5,202 digital images from 13 cancer types. Resulting TIL maps were correlated with TCGA molecular data, relating TIL content to survival, tumor subtypes, and immune profiles.
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7、Perspective on Oncogenic Processes at the End of the Beginning of Cancer Genomic

摘要:The Cancer Genome Atlas (TCGA) has catalyzed systematic characterization of diverse genomic alterations underlying human cancers. At this historic junction marking the completion of genomic characterization of over 11,000 tumors from 33 cancer types, we present our current understanding of the molecular processes governing oncogenesis. We illustrate our insights into cancer through synthesis of the findings of the TCGA PanCancer Atlas project on three facets of oncogenesis: (1) somatic driver mutations, germline pathogenic variants, and their interactions in the tumor; (2) the influence of the tumor genome and epigenome on transcriptome and proteome; and (3) the relationship between tumor and the microenvironment, including implications for drugs targeting driver events and immunotherapies. These results will anchor future characterization of rare and common tumor types, primary and relapsed tumors, and cancers across ancestry groups and will guide the deployment of clinical genomic sequencing. A synthesized view on oncogenic processes based on PanCancer Atlas analyses highlights the complex impact of genome alterations on the signaling and multi-omic profiles of human cancers as well as their influence on tumor microenvironment.
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8、Association of CDC25 phosphatase family polymorphisms with the efficacy/toxicity of platinum-based chemotherapy in Chinese advanced NSCLC patient

9、A forest-profit expectations dataset for New Zealand, 1990 - 2008 2012

摘要:從書:Motu working papers, 12,07; Motu working papers, Motu Economic and Public Policy Research Trust, 12,07 Online-Ressource (24 S.) graph. Darst.
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10、Biotechnology in China I : From Bioreaction to Bioseparation and Bioremediation 2009

摘要:從書:Advances in Biochemical Engineering, 113; Advances in biochemical engineering, biotechnology, 113. 1 online resource. In recent years, biotechnology research and development (R&D) in China has been receiving increasing attention from the world. With the open-door policy of the Chinese government, many international publications (for academia) and large market potential (for industry) constitute the two big reasons for the above phen- enon. Biotechnology has become one of the priorities in Mainland China for so- ing many important problems, such as food supply, health care, environment protection, and even energy. The central government has been implementing a c- ple of programs which cover a wide spectrum in basic research, high-tech devel- ment and industrialization, such as Basic Research Program (973 Plan), Hi-Tech R&D Program (863 Plan), Key Science & Technology Problem Solving Program (Gong-guan Plan), as well as the establishment of centers of excellence - Key Laboratories and Engineering Centers, etc. The funding from various local gove- ments and industry for R&D has also been increasing continuously. Biotechnology centers in Shenzhen, Shanghai and Beijing have been established. There are more than 400 universities, research institutes and companies and a total of over 20,000 researchers involved in biotechnology in the Mainland. The number of research papers published internationally and patent applications is also increasing rapidly. In addition, the huge market potential with about 1. 4 billion population, which is already open to the outside world, has provided numerous opportunities for int- national and domestic companies to invest in biotechnology, which pushes forward the biotechnology industrialization in China.
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11、Effect of Ionic Strength on the Interfacial Forces between Oil/Brine/Rock Interfaces: A Chemical Force Microscopy Study

12、Direct Experimental Evidence of Biomimetic Surfaces with Chemical Modifications Interfering with Adhesive Protein Adsorption

13、2018 IEEE Computer Society Annual Symposium on VLSI ISVLSI 2018 : proceedings : 9-11 July 2018, Hong Kong, Hong Kong 2018

14、Spatio-temporal variation of landfill gas in pilot-scale semi-aerobic and anaerobic landfills over 5 year

15、Spatio-temporal variation of landfill gas in pilot-scale semi-aerobic and anaerobic landfills over 5year

16、Clinical features of and risk factors for major depression with history of postpartum episodes in Han Chinese women: A retrospective study

摘要:Background: We sought to investigate the clinical features of and risk factors for recurrent major depression (MD) with history of postpartum episodes (PPD) in Han Chinese women and the differences between first-onset postpartum MD (MD that has its first lifetime depressive episode in the postpartum period) and first-onset non-postpartum MD (MD with history of PPD and has its first lifetime depressive episode in a period other than postpartum).Methods: Data were derived from the China, Oxford and Virginia Commonwealth University Experimental Research on Genetic Epidemiology (CONVERGE) study (N=6017 cases) and analyzed in two steps. We first examined the clinical features of and risk factors for MD patients with (N=981) or without (N=4410) a history of PPD. We then compared the differences between first-onset postpartum MD (N=583) and first-onset non-postpartum MD (N=398) in those with a history of PPD. Linear, logistic and multinomial logistic models were employed to measure the associations.Results: A history of PPD was associated with more guilt feelings, greater psychiatric comorbidity, higher neuroticism, earlier onset and more chronicity (OR 0.2-2.8). Severe premenstrual symptoms (PMS) and more childbirths increased the risk of PPD, as did a family history of MD, childhood sexual abuse, stressful life events and lack of social support (OR 1.1-1.3). In the MD with history of PPD subsample, first-onset postpartum MD was associated with fewer recurrent major depressive episodes, less psychiatric comorbidity, lower neuroticism, less severe PMS and fewer disagreements with their husbands (OR 0.5-0.8), but more childbirths (OR 1.2).Limitations: Data were obtained retrospectively through interview and recall bias may have affected the results.Conclusions: MD with history of PPD in Han Chinese women is typically chronic and severe, with particular risk factors including severe PMS and more childbirths. First-onset postpartum MD and first-onset non-postpartum MD can be partly differentiated by their clinical features and risk factors, but are not clearly distinctive.• This is the largest research on major depression (MD) in Han Chinese women in the world. • We focus on recurrent major depression with history of postpartum episodes (PPD). • 95.0% postpartum major depressive episodes start within 3 months after childbirth. • Severe premenstrual symptoms and more childbirths increase the risk for PPD. • Postpartum periods add to the chronicity and severity of MD with history of PPD.
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17、Suicidal Risk Factors of Recurrent Major Depression in Han Chinese Women

摘要:The relationship between suicidality and major depression is complex. Socio- demography, clinical features, comorbidity, clinical symptoms, and stressful life events are important factors influencing suicide in major depression, but these are not well defined. Thus, the aim of the present study was to assess the associations between the above-mentioned factors and suicide ideation, suicide plan, and suicide attempt in 6008 Han Chinese women with recurrent major depression (MD). Patients with any suicidality had significantly more MD symptoms, a significantly greater number of stressful life events, a positive family history of MD, a greater number of episodes, a significant experience of melancholia, and earlier age of onset. Comorbidity with dysthymia, generalized anxiety disorder (GAD), social phobia, and animal phobia was seen in suicidal patients. The present findings indicate that specific factors act to increase the likelihood of suicide in MD. Our results may help improve the clinical assessment of suicide risk in depressed patients, especially for women.
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18、Characterization and comparison of post-natal rat Achilles tendon-derived stem cells at different development stage

19、Design and synthesis of shape-persistent arylene-ethynylene covalent organic polyhedrons through alkyne metathesis and their fullerene-binding study 2014

摘要:1 online resource (186 pages .) Shape persistent 2-D macrocycles constructed via alkyne metathesis have been well explored in the past decade. However, 3-D Covalent Organic Polyhedrons (COPs) synthesized through alkyne metathesis have been rarely studied. The objective of the work described in this thesis is to explore the efficient assembly of 3-D shape-persistent covalent organic polyhedrons through dynamic alkyne metathesis and study the relationship of the COP structures and the geometry of their building blocks. The applications COPs in host-guest chemistry, specifically the interactions with fullerenes will be discussed.In Chapter 1, an overview of the current advances in alkyne metathesis reactions and their application in organic materials development, including design and synthesis of novel polymers, shape-persistent 2-D macrocycles, 3-D molecular cages and porous frameworks. In Chapter 2, the design and synthesis of a tetrameric COP with an unexpected D2h symmetry through one-step alkyne metathesis from a C3 symmetrical monomer is discussed. The COP structure was confirmed by the signal crystal X-ray diffraction. This COP exhibits high binding affinity with C70 over C60 (K C70/KC60> 1000). In Chapter 3, the synthesis of a dumbbell-shape tetrameric COP through alkyne metathesis is discussed. The formation of the tetrameric structure instead of entropically- favored dimeric one is investigated through computer modelling study. The cage also serves as a host for fullerenes. In Chapter 4, the relationship between cage structures and the geometry of monomers in the dynamic assembly process is discussed. A dimeric cage and its interlocked cages were obtained in one-pot alkyne metathesis starting from an extend C3 symmetry monomer. In Chapter 5, the design and synthesis of a series of pyrene based COPs are described. These COPs exhibit strong fluorescence and significant binding affinity with fullerenes. They can be transferred into cells, which have potential applications in diagnosis. A water soluble pyrene cage was also synthesized and its biological property study is undergoing.
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20、Systematic Comparative Evaluation of Methods for Investigating the TCRβ Repertoire

摘要:High-throughput sequencing has recently been applied to profile the high diversity of antibodyome/B cell receptors (BCRs) and T cell receptors (TCRs) among immune cells. To date, Multiplex PCR (MPCR) and 5』RACE are predominately used to enrich rearranged BCRs and TCRs. Both approaches have advantages and disadvantages; however, a systematic evaluation and direct comparison of them would benefit researchers in the selection of the most suitable method. In this study, we used both pooled control plasmids and spiked-in cells to benchmark the MPCR bias. RNA from three healthy donors was subsequently processed with the two methods to perform a comparative evaluation of the TCR β chain sequences. Both approaches demonstrated high reproducibility (R2 = 0.9958 and 0.9878, respectively). No differences in gene usage were identified for most V/J genes (>60%), and an average of 52.03% of the CDR3 amino acid sequences overlapped. MPCR exhibited a certain degree of bias, in which the usage of several genes deviated from 5』RACE, and some V-J pairings were lost. In contrast, there was a smaller rate of effective data from 5』RACE (11.25% less compared with MPCR). Nevertheless, the methodological variability was smaller compared with the biological variability. Through direct comparison, these findings provide novel insights into the two experimental methods, which will prove to be valuable in immune repertoire research and its interpretation.
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